3B Scientific Experiment kit: Electrophoresis and DNA Fragment Length Determination Lab Activity
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Restriction enzymes are enzymes that are capable to cleaving or cutting DNA molecules in very specific spots based on the DNA molecule’s sequence of bases Students will perform agarose electrophoresis on three DNA samples that have already been treated with restriction enzymes Students will learn about the process of DNA electrophoresis as well as about the techniques associated with the process such as setting up an agarose gel loading DNA samples in the agarose gel and staining the gel in order to visualize the DNA bands There is enough DNA to run 10 gels Needed but not supplied are agarose electrophoresis chambers power supplies and micropipettes capable of measuring 10µL
Description
(1)
Restriction enzymes are enzymes that are capable to cleaving or cutting DNA molecules in very specific spots based on the DNA molecule’s sequence of bases Students will perform agarose electrophoresis on three DNA samples that have already been treated with restriction enzymes Students will learn about the process of DNA electrophoresis as well as about the techniques associated with the process such as setting up an agarose gel loading DNA samples in the agarose gel and staining the gel in order to visualize the DNA bands There is enough DNA to run 10 gels Needed but not supplied are agarose electrophoresis chambers power supplies and micropipettes capable of measuring 10µL
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